A polymorphic (TG)n microsatellite in an intron of the canine tyrosine transaminase gene.

Source/description: PCR primers 1 and 2 were designed from the published human tyrosine transaminase sequence and used to amplify canine genomic DNA templates. Direct sequencing of the resulting 1.0 kb amplicons revealed 919±929 bp of canine sequence (GenBank accession no. L47165). The coding regions of the canine sequence (88 bp at the 59 end and the 3 bp at the 39 end) had 97% nucleotide sequence identity to corresponding sequences in exons H and I of the human tyrosine transaminase gene. The canine coding sequences were separated by an intron of 828±838 bp which contained a polymorphic (TG)n microsatellite. The canine nucleotide sequence was used to devise PCR primers 3 and 4 which annealed to sites closely flanking the microsatellite.